Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model

Table 4

Effects of the plant mixture aqueous extract on serum biochemical parameters in females.

Parameters	TN	TSAT	BC 200	BC 400	BC 800	SAT
TP (mg/dL)	0.90 ± 0.51	0.95 ± 0.46	0.88 ± 0.14	0.93 ± 0.57	0.93 ± 0.24	0.98 ± 0.19
AST (U/L)	71.80 ± 5.79	76.70 ± 6.34	74.50 ± 5.77	79.60 ± 5.41	80.40 ± 5.76	75.80 ± 4.69
ALT (U/L)	34.29 ± 2.62	31.06 ± 1.47	33.93 ± 2.52	40.49 ± 3.74	41.57 ± 2.48	39.3 ± 4.92
TB (mg/dL)	0.37 ± 0.09	0.66 ± 0.32	0.55 ± 0.20	0.90 ± 0.18	0.66 ± 0.29	0.46 ± 0.16
Creatinine (mg/dL)	0.99 ± 0.04	0.98 ± 0.04	0.94 ± 0.03	0.90 ± 0.03	0.92 ± 0.03	0.90 ± 0.02
Urea (mg/dL)	33.51 ± 1.04	35.58 ± 0.44	32.45 ± 0.23	30.08 ± 0.63	37.92 ± 0.21	36.20 ± 0.32
Uric acid (mg/dL)	2.89 ± 0.14	3.08 ± 0.09	2.94 ± 0.13	3.00 ± 0.33	2.92 ± 0.12	2.80 ± 0.07
TC (mg/dL)	54.07 ± 2.76	49.79 ± 5.38	65.63 ± 4.81^a	65.84 ± 3.62^a	43.82 ± 2.87	42.34 ± 4.69
TG (mg/dL)	36.13 ± 1.34	38.93 ± 1.58	40.08 ± 3.21	43.88 ± 4.30	36.91 ± 2.18	34.07 ± 1.90
HDL-C (mg/dL)	34.45 ± 1.68	27.89 ± 0.85	34.90 ± 2.80	42.37 ± 4.79	35.44 ± 2.18	30.21 ± 2.90

Each value represents the mean ± SEM, n = 5. TN: normal rats receiving distilled water (10 mL/kg); TSAT: normal satellite observed 14 more days after stopping every treatment; BC 200, BC 400, and BC 800: animals receiving the aqueous extract of the mixture of B. pilosa and C. citratus at the respective doses of 200, 400, and 800 mg/kg; SAT: satellite extract at the dose of 800 mg/kg observed 14 days after stopping every treatment. TP: total protein; ALT: alanine aminotransferase; AST: aspartate aminotransferase. TB: total bilirubin; TC: total cholesterol; HDL-C: high-density lipoprotein cholesterol; TG: triglycerides. ^a: significant difference from normal controls.