Toxicity of Glycyl-l-Prolyl-l-Glutamate Pseudotripeptides: Cytotoxic, Oxidative, Genotoxic, and Embryotoxic Perspectives

Table 3

The embryotoxic action potentials by GPEs on cell viabilities in cultured human pluripotent embryonal carcinoma NT2 cells. symbol presents a statistical difference from the control (-) group at the level of .

Groups	Concentrations	Cell viability (%, MTT)	Cell viability (%, LDH release)
Untreated cells		100	100
CPA		17.45 ± 3.28	13.20 ± 2.85
GPE	0.1 μM	98.60 ± 5.54	98.24 ± 7.12
	1 μM	97.40 ± 4.66	98.45 ± 6.54
	10 μM	97.14 ± 6.96	96.20 ± 5.87
	25 μM	98.10 ± 5.80	97.12 ± 7.91
	50 μM	95.42 ± 6.62	94.22 ± 6.97
	100 μM	93.78 ± 6.44	92.95 ± 8.24
GPE1	0.1 μM	97.48 ± 6.22	98.33 ± 4.33
	1 μM	98.11 ± 6.87	97.14 ± 5.18
	10 μM	94.35 ± 8.12	96.44 ± 5.23
	25 μM	92.46 ± 7.33	95.10 ± 5.88
	50 μM	90.81 ± 5.19	95.35 ± 6.13
	100 μM	89.50 ± 8.77	91.75 ± 6.34
GPE2	0.1 μM	99.30 ± 5.33	97.75 ± 7.42
	1 μM	98.25 ± 5.97	98.06 ± 6.90
	10 μM	97.65 ± 7.66	97.22 ± 8.12
	25 μM	97.21 ± 6.82	96.19 ± 7.41
	50 μM	94.22 ± 7.16	94.50 ± 7.79
	100 μM	91.56 ± 7.54	92.80 ± 7.72
GPE3	0.1 μM	98.44 ± 7.10	99.11 ± 7.89
	1 μM	99.26 ± 7.61	98.46 ± 8.41
	10 μM	97.43 ± 6.90	98.72 ± 8.33
	25 μM	94.82 ± 6.75	96.80 ± 7.15
	50 μM	92.75 ± 7.32	95.52 ± 6.02
	100 μM	93.35 ± 6.80	92.68 ± 5.90