The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study

Figure 1

RBCs as a transfer link in the influence of factors determining an excess of Fe²⁺ on clinical consequences in organisms. (a) A schematic representation of the influence of various factors determining excess Fe²⁺ on clinical consequences in the organism. Input includes : factors determining excess Fe²⁺; transfer function includes pathological changes in RBCs: , content of hemoglobin derivatives; , membrane stiffness; , cell morphology; , hemolysis; output includes : pathological clinical consequences. (b) Experimental design for pRBC lysate. The designation of pRBC lysate with iron is LFe²⁺ 1700. (c) Experimental design for pRBC suspension. The designation of pRBC suspension with iron is SFe²⁺ 1700. (d) Methods to study the action of excess Fe²⁺ on RBCs. AFM, atomic force microscopy; AFS, atomic force spectroscopy; KM, kinetic model; SP, spectrophotometry.