The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study

Figure 2

Increasing MetHb content with excessive content of free Fe²⁺. (a) Optical spectra of the LFe²⁺ 1700 sample, incubation time t_inc = 0, 15, 30, 45, 60, 90, and 120 minutes. On the right are the equations for calculating the concentrations of hemoglobin derivatives. (b) Increase in depending on the incubation time for the LFe²⁺ 1700 sample. Data are presented as box chart, from the 25th to the 75th percentile, whiskers are presented as mean ± SD, and the red square denotes mean. Dots are the experimental data. (c) Fitting results of the experimental data for the LFe²⁺ 0 and LFe²⁺ 1700 samples (t_inc = 0, 30, 60, and 120 min). Concentrations of hemoglobin derivatives are shown on each graph and were calculated by the nonlinear curve fitting method. Additionally, the R-square parameter is shown. (d, e) Dynamics of changes in the for different initial concentrations of free Fe²⁺ in dependence of time (0–1500 min (d) and 0–120 min (e)); on the graph axes, concentrations are in µM from the left and in % from the right. Initial concentrations of free Fe²⁺ are shown in different colors. (f) Increase in the as a function of the initial free Fe²⁺ concentration. Different colors correspond to different incubation times.