Research Article

The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study

Figure 3

Effects of free Fe2+ on the increase in MetHb content and the increase in polymorphisms. (a–c) Optical spectra and AFM images for the pRBC suspension (SFe2+ 0), scale 40 × 40 µm2. Incubation times were 1 h (a), 5 h (b), and 24 h (c). Color scales of heights are shown for the images. The is indicated on each graph. (d–f) Optical spectra and AFM images for the pRBC suspension after exposure to Fe2+ (SFe2+ 1700), scale 40 × 40 µm2. Incubation times were 1 h (d), 5 h (e), and 24 h (f). The is indicated on each graph. Color scales of heights are shown for the images.