The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study

Figure 4

Typical forms of pRBCs after free Fe²⁺ exposure. (a) 3D AFM images of typical cell shapes after exposure of pRBCs to free Fe²⁺; color scales of cell heights are shown. On the right of each cell, the profile and its size are shown as the mean ± SD. (b, c) Histograms of the dynamics of changes in the percentage of each cell form for SFe²⁺ 0 and SFe²⁺ 1700, t_inc = 1, 5, and 24 (h). On the right is the color coding for the different cell shapes. (d) Histogram of the dynamics of changes in the percentage of each cell form after 24 hours for different concentrations of free iron. On the right is the color coding for the different cell shapes.