Acute and Subchronic Toxicity Study of Flavonoid Rich Extract of Glycyrrhiza glabra (GutGard®) in Sprague Dawley Rats

Table 3

Effect of GutGard on haematological parameters of male rats during 90 D oral toxicity study.

Parameter	Dose (mg/kg)
	0	0 (Rev)^$	250	500	1000	1000 (Rev)^$
Hb (g/dL)	14.99 ± 0.61	16.26 ± 0.62	15.53 ± 1.29	15.75 ± 0.89	14.79 ± 0.70	16.64 ± 0.66
RBC × 10^6 (/μL)	8.54 ± 0.51	9.67 ± 0.34	8.59 ± 0.87	9.12 ± 0.63	7.97 ± 0.37	9.51 ± 0.69
HCT (%)	41.99 ± 2.03	47.62 ± 1.61	43.42 ± 3.66	45.29 ± 3.18	41.06 ± 1.86	47.38 ± 2.98
MCV (fL)	49.22 ± 1.36	49.26 ± 1.76	50.68 ± 1.98	49.67 ± 1.82	51.55 ± 1.50	49.86 ± 1.86
MCH (pg)	17.59 ± 0.55	16.82 ± 0.56	18.16 ± 0.75	17.31 ± 1.24	18.57 ± 0.60	17.56 ± 0.69
MCHC (g/dL)	35.73 ± 0.44	34.10 ± 0.64	35.84 ± 0.72	34.86 ± 2.00	36.01 ± 0.54	35.22 ± 1.24
PLTS × 10^3(/μL)	319.50 ± 118.44	440.20 ± 73.57	411.50 ± 61.47	416.10 ± 90.78	423.20 ± 80.72	366.40 ± 33.50
WBC × 10^3 (/μL)	8.57 ± 3.35	9.50 ± 1.78	13.65 ± 4.48	12.08 ± 4.16	13.31 ± 5.17	9.56 ± 2.82
Neutrophils (%)	21.40 ± 4.27	21.20 ± 3.70	20.40 ± 4.12	21.90 ± 3.70	20.30 ± 4.45	21.40 ± 4.51
Lymphocytes (%)	74.90 ± 3.84	75.00 ± 3.39	76.50 ± 4.14	75.20 ± 3.39	76.10 ± 4.98	75.40 ± 4.04
Eosinophils (%)	1.30 ± 0.67	1.20 ± 0.84	0.70 ± 0.82	0.80 ± 0.92	1.20 ± 0.92	1.00 ± 1.00
Monocytes (%)	2.40 ± 0.52	2.60 ± 0.55	2.40 ± 0.70	2.10 ± 0.57	2.40 ± 0.70	2.20 ± 0.84
Basophils (%)	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
PT (sec)	14.90 ± 3.21	14.80 ± 3.56	14.60 ± 3.10	14.70 ± 2.75	15.10 ± 3.28	15.40 ± 3.44

Mean ± SD (n = 10); ^$n = 5. Hb: hemoglobin; RBC: red blood corpuscles; HCT: hematocrit; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; WBC: white blood corpuscles; N: neutrophils; L: lymphocytes; E: eosinophils; M: monocytes; B: basophils; PT: prothrombin time; PLTS: platelets; Rev: reversal.  = significant at 95% level of confidence ;  = significant at 99% level of confidence .