Acute and Subchronic Toxicity Study of Flavonoid Rich Extract of Glycyrrhiza glabra (GutGard®) in Sprague Dawley Rats

Table 4

Effect of GutGard on haematological parameters of female rats during 90 D oral toxicity study.

Parameter	Dose (mg/kg)
	0	0 (Rev)^$	250	500	1000	1000 (Rev)^$
Hb (g/dL)	16.29 ± 1.56	15.64 ± 0.29	16.10 ± 2.08	15.78 ± 2.08	15.34 ± 0.75	16.02 ± 1.51
RBC × 10^6 (/μL)	8.56 ± 0.45	8.47 ± 0.40	8.54 ± 0.95	8.30 ± 1.05	8.12 ± 0.59	8.46 ± 0.95
HCT (%)	44.45 ± 3.92	43.70 ± 1.57	44.17 ± 5.92	42.89 ± 4.99	42.01 ± 3.18	43.98 ± 4.45
MCV (fL)	51.89 ± 2.24	51.62 ± 0.73	51.63 ± 1.50	51.71 ± 1.94	51.76 ± 1.82	52.04 ± 1.17
MCH (pg)	19.00 ± 1.17	18.48 ± 0.58	18.84 ± 0.66	19.03 ± 0.90	18.91 ± 0.81	18.98 ± 0.56
MCHC (g/dL)	36.64 ± 1.12	35.80 ± 0.77	36.46 ± 0.60	36.78 ± 0.77	36.58 ± 1.44	36.48 ± 0.37
PLTS × 10^3(/μL)	336.60 ± 88.50	360.20 ± 70.46	363.80 ± 93.03	392.90 ± 72.10	422.80 ± 55.47	335.80 ± 79.33
WBC × 10^3 (/μL)	9.74 ± 3.85	7.32 ± 5.28	7.97 ± 2.88	9.73 ± 3.93	15.73 ± 5.02	4.38 ± 0.86
Neutrophils (%)	22.50 ± 4.30	20.80 ± 3.27	21.20 ± 4.37	20.90 ± 4.15	21.40 ± 5.30	21.20 ± 4.21
Lymphocytes (%)	74.20 ± 3.58	75.80 ± 2.59	75.80 ± 4.21	76.10 ± 3.51	75.80 ± 5.03	75.80 ± 3.90
Eosinophils (%)	0.90 ± 0.74	1.00 ± 0.71	0.70 ± 0.67	0.90 ± 0.88	0.60 ± 0.70	0.80 ± 0.84
Monocytes (%)	2.40 ± 0.70	2.40 ± 0.55	2.30 ± 0.48	2.10 ± 0.57	2.20 ± 0.63	2.20 ± 0.84
Basophils (%)	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
PT (sec)	15.10 ± 3.07	15.40 ± 3.85	15.60 ± 3.44	15.40 ± 3.06	15.60 ± 2.95	14.80 ± 4.32

Mean ± SD (n = 10); ^$n = 5. Hb: hemoglobin; RBC: red blood corpuscles; HCT: hematocrit; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; WBC: white blood corpuscles; N: neutrophils; L: lymphocytes; E: eosinophils; M: monocytes; B: basophils; PT: prothrombin time; PLTS: platelets; Rev: reversal.  = significant at 95% level of confidence ;  = significant at 99% level of confidence .