Acute and Subchronic Oral Toxicity Evaluation of Herbal Formulation: Piper crocatum Ruiz and Pav., Typhonium flagelliforme (Lodd.) Blume, and Phyllanthus niruri L. in Sprague–Dawley Rats

Table 3

Hematological parameters of female SD rats fed exposed to SKM product (22.5; 45; 90 mg/kg b.w.) by oral route and control rats during the 90-day followed by a 28-day recovery period. Values are mean ± SE.

Parameters	Unit	Day 90 (±SEM)				Day 118 (±SEM)		Reference range [13, 14]
		Groups
		Control	22.5 mg/kg BW	45 mg/kg BW	90 mg/kg BW	Control	90 mg/kg BW
		n = 7	n = 9	n = 7	n = 7	n = 5	n = 3
RBC	10^4/μL	630.71 ± 30.74	702.44 ± 26.98	650.57 ± 20.41	590.00 ± 40.65	696.80 ± 5.39	678.67 ± 33.14	570.00–900.00
WBC	10^2/μL	59.14 ± 10.96	80.89 ± 11.31^b	38.43 ± 3.46	52.43 ± 5.55	70.4 ± 8.29	63 ± 11.60	20.00–115.00
Hb	g/dL	12.67 ± 0.51	13.90 ± 0.57	12.99 ± 0.36	11.44 ± 0.79	13.90 ± 0.26	12.80 ± 0.61	11.00–17.00
HCT	%	36.37 ± 1.77	40.44 ± 1.46^a	38.32 ± 1.11	33.2 ± 2.31	40.38 ± 0.64	38.37 ± 1.94	39.00–55.00
MCV	fL	57.69 ± 0.35	57.63 ± 0.32	58.94 ± 0.46	56.24 ± 0.79	57.96 ± 0.58	56.56 ± 0.54	55.00–65.00
MCH	Pg	20.16 ± 0.27	19.78 ± 0.28	19.99 ± 0.21	19.40 ± 0.34	19.96 ± 0.26	18.87 ± 0.07	17.00–22.00
MCHC	g/dL	34.91 ± 0.38	34.33 ± 0.42	33.89 ± 0.22	34.49 ± 0.16	34.44 ± 0.34	33.37 ± 0.47	28.00–34.00
Platelet	10^4/μL	27.56 ± 5.13	51.26 ± 7.72	20.51 ± 2.44	36.03 ± 6.71	36.54 ± 7.94	27.90 ± 7.44	70.00–150.00

^aSignificant differences compared with 90 mg/kg b.w. (day 90); ^bsignificant differences compared with 45 mg/kg b.w. (day 90).