Acetylsalicylic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Mesenchymal Stem Cells and Regeneration of Alveolar Bone in Experimental Periodontitis Rats

Figure 2

The effects of ASA on cell proliferation and osteogenic differentiation in vitro. (a) Proliferation assessment of hDPMSCs after culture with a series of concentrations of ASA (0, 25, 50, 75, 100, 150, and 200 µg/mL) for 24 h, 48 h, and 72 h. 48 h: 0 µg/ml vs. 25 µg/ml  = 0.4285; 0 µg/ml vs. 50 µg/ml  = 0.9817; 0 µg/ml vs. 75 µg/ml  = 0.9999; 0 µg/ml vs. 100 µg/ml  = 0.9237; 0 µg/ml vs. 150 µg/ml  = 0.0196 n = 3 for all the groups. (b and c) Staining and quantitative detection of alkaline phosphatase (ALP) activity in hDPMSCs after culture in ASA under osteoinductive medium for 7 and 14 days. n = 3 for all the groups. (d and e) Alizarin red staining of the hDPMSCs under incubation of ASA in osteoinductive medium for 21 days. n = 3 for all the groups. (f) Western blot analysis for the expression level of RUNX2 and OPN protein after ASA treatment for 7 days. (g) Quantitative analysis of the protein levels of RUNX2 and OPN. n = 3 for all the groups. All values represent the mean ± SD of triplicate experiments.  < 0.05;  < 0.01;  < 0.001.

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