Acetylsalicylic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Mesenchymal Stem Cells and Regeneration of Alveolar Bone in Experimental Periodontitis Rats

Figure 4

The evaluation of ASA on hDPMSC-based on alveolar bone repair and regeneration in periodontitis rats. (a) Alveolar bone loss analysis at 4 weeks after transplantation. The distance from the CEJ to the ABC at 6 sites of the second molar was examined to measure a bone resorption in all five groups. Bar = 1 mm; control vs. periodontitis  < 0.0001; periodontitis vs. ASA  = 0.0359; periodontitis vs. hDPMSCs  = 0.0036; hDPMSCs vs. ASA-hDPMSCs  = 0.0012; n = 5. (b) The HE staining results. (c) Immunohistochemical staining and quantitative analysis of the number of OPG; control vs. ASA  = 0.1792; periodontitis vs. ASA  = 0.0084; DPMSCs vs. ASA-DPMSCs  = 0.0005; n = 5. (d) Immunohistochemical staining and quantitative analysis of the number of RANKL. Control vs. periodontitis  < 0.0001; periodontitis vs. ASA  = 0.0010; ASA vs. ASA-DPMSCs  = 0.0041. n = 4 for all the groups. (AB = alveolar bone, PDL = periodontal ligament, R = tooth root). Bar = 50 µm/200 µm. The results were expressed as the mean ± SD.

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