Modelling Skeletal Muscle Ageing and Repair In Vitro

Figure 2

Young and aged muscle cells show similar proliferation response. EdU incorporation in mouse C2C12 and human skeletal muscle cells (EdU + cells are represented in green (arrow) and Hoechst + cells are represented in blue) indicating DNA synthesis in S-phase of cell cycle before (CTRL) and after BaCl₂-induced injury. No EdU + cells were detected in old myotubes at the end of the regeneration period (END DIFF, n.d.) (a). Significant increase in EdU + cells in culture was reached by the end of proliferation of the regeneration period (END PROL) in each cell line (n = 3; ) (b). Total nuclei number in myotubes derived from mouse (C2C12) and human young and aged myoblasts after injury (0 h) and the regenerative period (END PROL and END DIFF). Treatment with BaCl₂ retains precursor cells and initiates regenerative response in cultures. Significant increase in the total nuclei number was reached by the end of proliferation of the regeneration period (END PROL) in each cell line (n = 3; , , and (c). Statistical analysis performed by one-way ANOVA; all error bars represent standard error of the mean (SEM); scale bars 100 μm; circles indicate individual data points of technical replicates.

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(b)

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