Figure 2: Phosphatidylserine externalisation (PS+) measured by FITC-Annexin V binding in both infected erythrocytes (iRBC) and their neighbouring noninfected erythrocytes (nRBC). iRBC were identified by the uptake of the cell-permeable DNA-binding dye dihydroethidium. An increase in PS externalisation can be seen by comparing an uninfected erythrocyte culture (a), to a parasite culture (b), both maintained at 37°C, and a heat-stressed parasite culture maintained at 40°C for 48 hours (c).