Comparative Evaluation of Permissiveness to Dengue Virus Serotype 2 Infection in Primary Rodent Macrophages

Table 1

Percentage of infected and positive TUNEL cultured macrophages from different rodents.

		0.1 MOI						1 MOI
	MOCK	24 h		48 h		72 h		24 h		48 h		72 h
	TUNEL	DENV+	TUNEL	DENV+	TUNEL	DENV+	TUNEL	DENV+	TUNEL	DENV+	TUNEL	DENV+	TUNEL
BALB/c	4.0	13.2	5.2	79.8	8.2	81.4	5.0	43.4	88.6	100	91	86.8	99.2
ICR	13.0	26.6	9.2	21.8	17.0	22.6	13.6	13.2	4.6	25.6	23.6	34.2	17.0
NIH	8.0	5.8	5.0	12.2	9.4	15.6	31.0	9.2	9.4	13.8	29.2	17.2	9.2
Rat	2.0	24.4	4.6	17.8	5.2	23	7.4	20.6	5.6	13.6	4.2	8.0	6.3
Guinea pig	5.2	5.2	3.2	12.4	8.6	24.4	4.6	5.2	6.0	17.8	8.0	29.6	3.8
Hamster	7.0	13.4	12.0	32.8	34.8	8.6	33.2	6.4	3.0	5.4	17.0	10	15.2

The data represent the percentage of DENV antigen positive over the total of cells counted. Note that only 43.4% of the cells were positive for viral antigen in BALB/c mouse macrophage infected with 1 MOI, 24 hours p.i., but almost all the cells presented DNA degradation. Two animals were used from each species; two groups of independent cells were seeded for each animal and two replicates were done for each of them in immunocytochemistry and TUNEL assays (𝑛=8).