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Journal of Tropical Medicine
Volume 2012, Article ID 956875, 10 pages
http://dx.doi.org/10.1155/2012/956875
Research Article

Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus

1LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, 94340 Orizaba, VER, Mexico
2Centro de Investigaciones Biomédicas, Universidad Veracruzana, 91190 Xalapa, VER, Mexico
3Laboratorio de Biología Molecular, Departamento de Infectómica y Patogénesis Molecular, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional, 07360 México, DF, Mexico
4Laboratorio Estatal de Salud Pública, Secretaría de Salud de Veracruz, 91697 Xalapa, VER, Mexico

Received 2 June 2012; Revised 14 September 2012; Accepted 18 October 2012

Academic Editor: Maria Aparecida Shikanai Yasuda

Copyright © 2012 Laura Mónica Álvarez-Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. CENAVECE, “Dengue; distribución del vector,” http://www.cenave.gob.mx/Dengue.
  2. D. J. Gubler, “The global pandemic of dengue/dengue haemorrhagic fever: current status and prospects for the future,” Annals of the Academy of Medicine Singapore, vol. 27, no. 2, pp. 227–234, 1998. View at Google Scholar · View at Scopus
  3. S. B. Halstead and E. J. O'Rourke, “Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody,” Journal of Experimental Medicine, vol. 146, no. 1, pp. 201–217, 1977. View at Google Scholar · View at Scopus
  4. S. B. Halstead, “Pathogenisis of dengue: challenges to molecular biology,” Science, vol. 239, no. 4839, pp. 476–481, 1988. View at Google Scholar · View at Scopus
  5. J. T. Roehrig, “Antigenic structure of flavivirus proteins,” Advances in Virus Research, vol. 59, pp. 141–175, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. B. D. Lindenbach and C. M. Rice, “Molecular biology of flaviviruses,” Advances in Virus Research, vol. 59, pp. 23–61, 2003. View at Publisher · View at Google Scholar · View at Scopus
  7. E. Harris, K. L. Holden, D. Edgil, C. Polacek, and K. Clyde, “Molecular biology of flaviviruses,” Novartis Foundation Symposium, vol. 40, no. 71-73, pp. 251–253, 2006. View at Google Scholar · View at Scopus
  8. C. A. X. Costa, T. H. S. Fonseca, F. M. S. Oliveira, J. F. G. Santos, M. A. Gomes, and M. V. Caliari, “Influence of inflammation on parasitism and area of experimental amoebic liver abscess: an immunohistochemical and morphometric study,” Parasites and Vectors, vol. 4, p. 27, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. L. Lazaro-Olán, G. Mellado-Sánchez, J. García-Cordero et al., “Analysis of antibody response in human dengue patients from the Mexican coast using recombinant antigens,” Vector-Borne and Zoonotic Diseases, vol. 8, no. 1, pp. 69–79, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. D. Hu, B. Di, X. Ding et al., “Kinetics of non-structural protein 1, IgM and IgG antibodies in dengue type 1 primary infection,” Virology Journal, vol. 2, no. 8, pp. 47–51, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. A. L. Rothman, “Dengue: defining protective versus pathologic immunity,” The Journal of Clinical Investigation, vol. 113, no. 7, pp. 946–951, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. A. Mathew and A. L. Rothman, “Understanding the contribution of cellular immunity to dengue disease pathogenesis,” Immunological Reviews, vol. 225, no. 1, pp. 300–313, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. R. Appanna, L. H. Tan, L. L. C. See, L. T. Phoay, J. Vadivelu, and S. Devi, “Cross-reactive T-cell responses to the nonstructural regions of dengue viruses among dengue fever and dengue hemorrhagic fever patients in Malaysia,” Clinical and Vaccine Immunology, vol. 14, no. 8, pp. 969–977, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. S. J. Balsitis, K. L. Williams, R. Lachica et al., “Lethal antibody enhancement of dengue disease in mice is prevented by Fc modification,” PLoS Pathogens, vol. 6, no. 2, Article ID e1000790, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. J. J. Schlesinger, M. W. Brandriss, and E. E. Walsh, “Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1,” Journal of General Virology, vol. 68, no. 3, pp. 853–857, 1987. View at Google Scholar · View at Scopus
  16. B. Falgout, M. Bray, J. J. Schlesinger, and C. J. Lai, “Immunization of mice with recombinant vaccinia virus expressing authentic dengue virus nonstructural protein NS1 protects against lethal dengue virus encephalitis,” Journal of Virology, vol. 64, no. 9, pp. 4356–4363, 1990. View at Google Scholar · View at Scopus
  17. S. M. Costa, A. S. Azevedo, M. V. Paes, F. S. Sarges, M. S. Freire, and A. M. B. Alves, “DNA vaccines against dengue virus based on the ns1 gene: the influence of different signal sequences on the protein expression and its correlation to the immune response elicited in mice,” Virology, vol. 358, no. 2, pp. 413–423, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. J. J. Schlesinger, M. Foltzer, and S. Chapman, “The Fc portion of antibody to yellow fever virus NS1 is a determinant of protection against YF encephalitis in mice,” Virology, vol. 192, no. 1, pp. 132–141, 1993. View at Publisher · View at Google Scholar · View at Scopus
  19. A. K. I. Falconar, “Antibody responses are generated to immunodominant ELK/KLE-type motifs on the nonstructural-1 glycoprotein during live dengue virus infections in mice and humans: implications for diagnosis, pathogenesis, and vaccine design,” Clinical and Vaccine Immunology, vol. 14, no. 5, pp. 493–504, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. C. F. Lin, S. W. Wan, M. C. Chen et al., “Liver injury caused by antibodies against dengue virus nonstructural protein 1 in a murine model,” Laboratory Investigation, vol. 88, no. 10, pp. 1079–1089, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. H. J. Cheng, H. Y. Lei, C. F. Lin et al., “Anti-dengue virus nonstructural protein 1 antibodies recognize protein disulfide isomerase on platelets and inhibit platelet aggregation,” Molecular Immunology, vol. 47, no. 2-3, pp. 398–406, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. A. P. Durbin and S. S. Whitehead, “Next-generation dengue vaccines: novel strategies currently under development,” Viruses, vol. 3, no. 10, pp. 1800–1814, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. M. M. B. Moreno-Altamirano, F. J. Sánchez-García, I. López-Martínez et al., “Improving dengue virus diagnosis in rural areas of Mexico,” Dengue Bulletin, vol. 33, no. 1, pp. 87–94, 2009. View at Google Scholar
  24. K. I. Yamada, T. Takasaki, M. Nawa, and I. Kurane, “Virus isolation as one of the diagnostic methods for dengue virus infection,” Journal of Clinical Virology, vol. 24, no. 3, pp. 203–209, 2002. View at Publisher · View at Google Scholar · View at Scopus
  25. Y. L. Lai, Y. K. Chung, H. C. Tan et al., “Cost-effective real-time reverse transcriptase PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus,” Journal of Clinical Microbiology, vol. 45, no. 3, pp. 935–941, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Alcon, A. Talarmin, M. Debruyne, A. Falconar, V. Deubel, and M. Flamand, “Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections,” Journal of Clinical Microbiology, vol. 40, no. 2, pp. 376–381, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. T. T. T. Nga, K. T. D. Thai, L. P. Hoang et al., “Evaluation of two rapid immunochromatographic assays for diagnosis of dengue among Vietnamese febrile patients,” Clinical and Vaccine Immunology, vol. 14, no. 6, pp. 799–801, 2007. View at Publisher · View at Google Scholar · View at Scopus
  28. E. A. Hunsperger, S. Yoksan, P. Buchy et al., “Evaluation of commercially available anti-dengue virus immunoglobulin M tests,” Emerging Infectious Diseases, vol. 15, no. 3, pp. 436–440, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. J. Li, S. P. Lim, D. Beer et al., “Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries,” The Journal of Biological Chemistry, vol. 280, no. 31, pp. 28766–28774, 2005. View at Publisher · View at Google Scholar · View at Scopus
  30. A. López-Monteon, A. Ramos-Ligonio, L. Pérez-Castillo, P. Talamás-Rohana, and J. L. Rosales-Encina, “Specific antibody immune response against the parasitic portion of a glutathione-S-transferase fusion protein,” The FASEB Journal, vol. 17, no. 6, pp. 621–627, 2003. View at Publisher · View at Google Scholar · View at Scopus
  31. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  32. J. Renart, J. Reiser, and G. R. Stark, “Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure,” Proceedings of the National Academy of Sciences of the United States of America, vol. 76, no. 7, pp. 3116–3120, 1979. View at Google Scholar · View at Scopus
  33. H. Towbin, T. Staehelin, and J. Gordon, “Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 1979,” Biotechnology, vol. 24, pp. 145–149, 1992. View at Google Scholar · View at Scopus
  34. R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A. V. Vorndam, “Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction,” Journal of Clinical Microbiology, vol. 30, no. 3, pp. 545–551, 1992. View at Google Scholar · View at Scopus
  35. J. Escobar-Mesa and H. Gómez-Dantés, “Dengue fever transmission in Veracruz, Mexico: an ecological approach to disease control,” Salud Publica de Mexico, vol. 45, no. 1, pp. 43–53, 2003. View at Google Scholar · View at Scopus
  36. K. Bessoff, E. Phoutrides, M. Delorey, L. N. Acosta, and E. Hunsperger, “Utility of a commercial nonstructural protein 1 antigen capture kit as a dengue virus diagnostic tool,” Clinical and Vaccine Immunology, vol. 17, no. 6, pp. 949–953, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. T. N. T. Tran, P. J. de Vries, L. P. Hoang et al., “Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood,” BMC Infectious Diseases, vol. 6, p. 13, 2006. View at Publisher · View at Google Scholar · View at Scopus
  38. H. Xu, B. Di, Y. X. Pan et al., “Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: implications for early diagnosis and serotyping of dengue virus infections,” Journal of Clinical Microbiology, vol. 44, no. 8, pp. 2872–2878, 2006. View at Publisher · View at Google Scholar · View at Scopus
  39. V. Churdboonchart, N. Bhamarapravati, S. Peampramprecha, and S. Sirinavin, “Antibodies against dengue viral proteins in primary and secondary dengue hemorrhagic fever,” American Journal of Tropical Medicine and Hygiene, vol. 44, no. 5, pp. 481–493, 1991. View at Google Scholar · View at Scopus
  40. K. Valdes, M. Alvarez, M. Pupo, S. Vazquez, R. Rodriguez, and M. G. Guzman, “Human dengue antibodies against structural and nonstructural proteins,” Clinical and Diagnostic Laboratory Immunology, vol. 7, no. 5, pp. 856–857, 2000. View at Publisher · View at Google Scholar · View at Scopus
  41. L. M. De C Côrtes, O. M. Barth, J. R. Pantoja, and C. R. Alves, “Comparative immunological recognition of proteins from New Guinea “C” dengue virus type 2 prototype and from a dengue virus type 2 strain isolated in the state of Rio de Janeiro, Brazil,” Memorias do Instituto Oswaldo Cruz, vol. 98, no. 3, pp. 331–334, 2003. View at Google Scholar · View at Scopus
  42. TDR/WHO, Dengue Diagnostics: Proceedings of an International Workshop 4–6 October 2004, TDR/WHO, Geneva, Switzerland, 2005, http://apps.who.int/tdr/publications/tdr-research-publications/dengue-diagnostics-proceedings/pdf/dengue_diagnostics.pdf.