In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations
Figure 4
Western blot to detect GLUT1 transporter protein expression in homogenates from normal placental villi, cultured for 24 hours (a) and 48 hours (b). Actin presence was also detected as a positive control for protein content in samples. GLUT1 expression was evaluated with the “Scion Image for Windows” program that measured the area units marked. WCN: without culture normal placental villi (positive control); N: normal placental villi; N + T: normal placental villi cocultured with T. cruzi; G: normal placental villi cultured under high glucose media; G + T: normal placental villi cocultured with T. cruzi under high glucose media. Values shown are the mean ± SE. *Significantly different from the control (; ; ANOVA test).
