Table of Contents Author Guidelines Submit a Manuscript
Journal of Tropical Medicine
Volume 2012 (2012), Article ID 969243, 8 pages
http://dx.doi.org/10.1155/2012/969243
Research Article

In Vitro Infection of Trypanosoma cruzi Causes Decrease in Glucose Transporter Protein-1 (GLUT1) Expression in Explants of Human Placental Villi Cultured under Normal and High Glucose Concentrations

1Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5016 Córdoba, Argentina
2Cátedra de Histología, Embriología y Genética, IICSHUM, Universidad Nacional de La Rioja, 5300 La Rioja, Argentina
3Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 35053 Zhunan Town, Miaoli County, Taiwan

Received 2 June 2011; Accepted 15 July 2011

Academic Editor: Ulrike Kemmerling

Copyright © 2012 Luciana Mezzano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. L. Bittencourt, “Congenital Chagas disease,” American Journal of Diseases of Children, vol. 130, no. 1, pp. 97–103, 1976. View at Google Scholar · View at Scopus
  2. I. Oliveira, F. Torrico, J. Muñoz, and J. Gascon, “Congenital transmission of Chagas disease: a clinical approach,” Expert Review of Anti-Infective Therapy, vol. 8, no. 8, pp. 945–956, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  3. M. Ming, M. Chuenkova, E. Ortega-Barria, and M. E. A. Pereira, “Mediation of Trypanosoma cruzi invasion by sialic acid on the host cell and trans-sialidase on the trypanosome,” Molecular and Biochemical Parasitology, vol. 59, no. 2, pp. 243–252, 1993. View at Publisher · View at Google Scholar · View at Scopus
  4. S. Schenkman, N. W. Andrews, V. Nussenzweig, and E. S. Robbins, “Trypanosoma cruzi invade a mammalian epithelial cell in a polarized manner,” Cell, vol. 55, no. 1, pp. 157–165, 1988. View at Google Scholar · View at Scopus
  5. I. Tardieux, M. H. Nathanson, and N. W. Andrews, “Role in host cell invasion of Trypanosoma cruzi-induced cytosolic-free Ca2+ transients,” Journal of Experimental Medicine, vol. 179, no. 3, pp. 1017–1022, 1994. View at Google Scholar · View at Scopus
  6. A. G. Todorov, M. Einicker-Lamas, S. L. de Castro, M. M. Oliveira, and A. Guilherme, “Activation of host cell phosphatidylinositol 3-kinases by Trypanosoma cruzi infection,” Journal of Biological Chemistry, vol. 275, no. 41, pp. 32182–32186, 2000. View at Google Scholar · View at Scopus
  7. M. J. Sartori, L. Mezzano, S. Lin, S. Muñoz, and S. P. de Fabro, “Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells,” Tropical Medicine & International Health, vol. 8, no. 9, pp. 832–839, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. M. J. Sartori, L. Mezzano, S. Lin, G. Repossi, and S. P. Fabro, “Cellular components and placental alkaline phosphatase in Trypanosoma cruzi infection,” Revista da Sociedade Brasileira de Medicina Tropical, vol. 38, supplement 2, pp. 87–91, 2005. View at Google Scholar
  9. L. Mezzano, M. J. Sartori, S. Lin, G. Repossi, and S. P. de Fabro, “Placental alkaline phosphatase (PLAP) study in diabetic human placental villi infected with Trypanosoma cruzi,” Placenta, vol. 26, no. 1, pp. 85–92, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  10. F. Nagajyothi, L. M. Weiss, D. L. Silver et al., “Trypanosoma cruzi utilizes the host low density lipoprotein receptor in invasion,” PLoS Neglected Tropical Diseases, vol. 5, no. 2, p. e953, 2011. View at Google Scholar
  11. R. G. Long, R. H. Albuquerque, A. Prata et al., “Response of plasma pancreatic and gastrointestinal hormones and growth hormone to oral and intravenous glucose and insulin hypoglycaemia in Chagas's disease,” Gut, vol. 21, no. 9, pp. 772–777, 1980. View at Google Scholar
  12. M. E. Guariento, E. Olga, A. Muscelli, and J. A. Gontijo, “Chronotropic and blood pressure response to oral glucose load in Chagas' disease,” Sao Paulo Medical Journal, vol. 112, no. 3, pp. 602–606, 1994. View at Google Scholar · View at Scopus
  13. J. C. Saldanha, V. M. Dos Santos, M. A. Dos Reis, D. F. Da Cunha, and V. P. A. Teixeira, “Morphologic and morphometric evaluation of pancreatic islets in chronic Chagas' disease,” Revista do Hospital das Clinicas de Faculdade de Medicina da Universidade de Sao Paulo, vol. 56, no. 5, pp. 131–138, 2001. View at Google Scholar · View at Scopus
  14. V. M. Dos Santos, M. A. de Lima, M. Cabrine-Santos et al., “Functional and histopathological study of the pancreas in hamsters (Mesocricetus auratus) infected and reinfected with Trypanosoma cruzi,” Parasitology Research, vol. 94, no. 2, pp. 125–133, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  15. H. B. Tanowitz, B. Amole, D. Hewlett, and M. Wittner, “Trypanosoma cruzi infection in diabetic mice,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 82, no. 1, pp. 90–93, 1988. View at Google Scholar · View at Scopus
  16. V. M. Dos Santos, S. F. Da Cunha, V. P. Teixeira et al., “Frequency of diabetes mellitus and hyperglycemia in chagasic and non-chagasic women,” Revista da Sociedade Brasileira de Medicina Tropical, vol. 32, no. 5, pp. 489–496, 1999. View at Google Scholar · View at Scopus
  17. H. Li, Y. Gu, Y. Zhang, M. J. Lucas, and Y. Wang, “High glucose levels down-regulate glucose transporter expression that correlates with increased oxidative stress in placental trophoblast cells in vitro,” Journal of the Society for Gynecologic Investigation, vol. 11, no. 2, pp. 75–81, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  18. M. U. Baumann, S. Deborde, and N. P. Illsley, “Placental glucose transfer and fetal growth,” Endocrine, vol. 19, no. 1, pp. 13–22, 2002. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  19. T. Jansson, M. Wennergren, and T. L. Powell, “Placental glucose transport and GLUT 1 expression in insulin-dependent diabetes,” American Journal of Obstetrics and Gynecology, vol. 180, no. 1, part 1, pp. 163–168, 1999. View at Publisher · View at Google Scholar · View at Scopus
  20. N. P. Illsley, “Glucose transporters in the human placenta,” Placenta, vol. 21, no. 1, pp. 14–22, 2000. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  21. K. Ogura, M. Sakata, M. Yamaguchi, H. Kurachi, and Y. Murata, “High concentration of glucose decreases glucose transporter-1 expression in mouse placenta in vitro and in vivo,” Journal of Endocrinology, vol. 160, no. 3, pp. 443–452, 1999. View at Google Scholar · View at Scopus
  22. T. Hahn, S. Barth, U. Weiss, W. Mosgoeller, and G. Desoye, “Sustained hyperglycemia in vitro down-regulates the GLUT1 glucose transport system of cultured human term placental trophoblast: a mechanism to protect fetal development?” FASEB Journal, vol. 12, no. 12, pp. 1221–1231, 1998. View at Google Scholar · View at Scopus
  23. N. W. Andrews and W. Colli, “Adhesion and interiorization of Trypanosoma cruzi in mammalian cells,” Journal of Protozoology, vol. 29, no. 2, pp. 264–269, 1982. View at Google Scholar · View at Scopus
  24. R. E. Fretes and S. P. de Fabro, “Trypanosoma cruzi: modification of alkaline phosphatase activity induced by trypomastigotes in cultured human placental villi,” Revista do Instituto de Medicina Tropical de Sao Paulo, vol. 32, no. 6, pp. 403–408, 1990. View at Google Scholar · View at Scopus
  25. S. Lin, M. J. Sartori, L. Mezzano, and S. P. de Fabro, “Placental alkaline phosphatase (PLAP) enzyme activity and binding to IgG in Chagas' disease,” Placenta, vol. 26, no. 10, pp. 789–795, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  26. O. H. Lowry, N. J. Rosebrough , A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar · View at Scopus
  27. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  28. P. Vannini, “Pregnancy and diabetes: physiopathological aspects,” Minerva Endocrinologica, vol. 19, no. 2, pp. 45–50, 1994. View at Google Scholar · View at Scopus
  29. P. Garner, “Type I diabetes mellitus and pregnancy,” The Lancet, vol. 346, no. 8968, pp. 157–161, 1995. View at Google Scholar · View at Scopus
  30. G. Desoye and E. Shafrir, “The human placenta in diabetic pregnancy,” Diabetes Reviews, vol. 4, no. 1, pp. 70–89, 1996. View at Google Scholar · View at Scopus
  31. J. F. Plouffe, J. Silva Jr., R. Fekety, and J. L. Allen, “Cell-mediated immunity in diabetes mellitus,” Infection & Immunity, vol. 21, no. 2, pp. 425–429, 1978. View at Google Scholar · View at Scopus
  32. J. Casey and C. Sturm Jr., “Impaired response of lymphocytes from non-insulin-dependent diabetics to staphage lysate and tetanus antigen,” Journal of Clinical Microbiology, vol. 15, no. 1, pp. 109–114, 1982. View at Google Scholar · View at Scopus
  33. S. Priotto, M. J. Sartori, G. Repossi, and M. A. Valentich, “Trypanosoma cruzi: participation of cholesterol and placental alkaline phosphatase in the host cell invasion,” Experimental Parasitology, vol. 122, no. 1, pp. 70–73, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  34. A. E. Fabro and G. Calzolari, “Increase of lipids in human chagasic placentas: cytochemical and biochemical study,” Revista de la Facultad de Ciencias Médicas de la Universidad Nacional de Córdoba, vol. 48, no. 1-2, pp. 25–32, 1990. View at Google Scholar · View at Scopus
  35. R. O. Calderón and S. P. de Fabro, “Trypanosoma cruzi: fusogenic ability of membranes from cultured epimastigotes in interaction with human syncytiotrophoblast,” Experimental Parasitology, vol. 56, no. 2, pp. 169–179, 1983. View at Google Scholar · View at Scopus
  36. N. P. Illsley, M. C. Sellers, and R. L. Wright, “Glycaemic regulation of glucose transporter expression and activity in the human placenta,” Placenta, vol. 19, no. 7, pp. 517–524, 1998. View at Publisher · View at Google Scholar · View at Scopus
  37. S. Hauguel-De Mouzon, M. Loizeau, and J. Girard, “Developmental expression of Glut1 glucose transporter and c-fos genes in human placental cells,” Placenta, vol. 15, no. 1, pp. 35–46, 1994. View at Google Scholar · View at Scopus
  38. S. Barth, T. Hahn, R. Zechner, and G. Desoye, “Prolonged hyperglycemia in vitro affects glucose transporter protein Glut 1 and glucose uptake in cultured term trophoblast cells,” Placenta, vol. 15, p. 4A, 1994. View at Google Scholar
  39. R. E. Fretes and S. P. de Fabro, “Human chagasic placental localization of enzymes associated to syncytiotrophoblast membrane,” Comunicaciones Biolo6gicas, vol. 9, pp. 51–59, 1990. View at Google Scholar
  40. M. J. Sartori, P. Pons, L. Mezzano, S. Lin, and S. P. de Fabro, “Trypanosoma cruzi infection induces microfilament depletion in human placenta syncytiotrophoblast,” Placenta, vol. 24, no. 7, pp. 767–771, 2003. View at Publisher · View at Google Scholar · View at Scopus
  41. S. Lin, M. J. Sartori, L. Mezzano, and S. P. de Fabro, “Epidermal growth factor (EGF) in the human placental infection with Trypanosoma cruzi,” Placenta, vol. 25, no. 4, pp. 283–286, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  42. Z. Salamon, S. Devanathan, I. D. Alves, and G. Tollin, “Plasmon-waveguide resonance studies of lateral segregation of lipids and proteins into microdomains (rafts) in solid-supported bilayers,” Journal of Biological Chemistry, vol. 280, no. 12, pp. 11175–11184, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  43. T. Sakyo, H. Naraba, H. Teraoka, and T. Kitagawa, “The intrinsic structure of glucose transporter isoforms Glut1 and Glut3 regulates their differential distribution to detergent-resistant membrane domains in nonpolarized mammalian cells,” FEBS Journal, vol. 274, no. 11, pp. 2843–2853, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  44. J. Duaso, G. Rojo, G. Cabrera et al., “Trypanosoma cruzi induces tissue disorganization and destruction of chorionic villi in an ex vivo infection model of human placenta,” Placenta, vol. 31, no. 8, pp. 705–711, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  45. C. D. Luján, M. F. Triquell, A. Sembaj, C. E. Guerrero, and R. E. Fretes, “Trypanosoma cruzi: productive infection is not allowed by chorionic villous explant from normal human placenta in vitro,” Experimental Parasitology, vol. 108, no. 3-4, pp. 176–181, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  46. M. F. Triquell, C. Díaz-Luján, H. Freilij, P. Paglini, and R. E. Fretes, “Placental infection by two subpopulations of Trypanosoma cruzi is conditioned by differential survival of the parasite in a deleterious placental medium and not by tissue reproduction,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 103, no. 10, pp. 1011–1018, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus