Research Article

Immunological Consequences of Antihelminthic Treatment in Preschool Children Exposed to Urogenital Schistosome Infection

Table 3

Potential identities of the proteins recognized.

BandProtein nameSpeciesNCBI accession no.Hit scoreMass

Putative glycogen phosphorylaseS. mansoni gi∣360045358 56494169
Surface proteinS. mansoni gi∣501209 182190732
~80 kDaMyosin heavy chainS. mansoni gi∣256086969 159171563
ActinS. mansoni gi∣256084607 13835652
Putative heat shock protein 70S. mansoni gi∣353229993 12570215
Hypothetical protein Smp_208030S. mansoni gi∣353231600 53333514

~100 kDaGPI-anchored surface glycoproteinS. mansoni gi∣256052078 129187956
Putative glycogen phosphorylaseS. mansoni gi∣360045358 10094169

The 2 bands on silver stain that matched those identified on immunoblots were excised and subjected to trypsin digestion before being analysed by mass spectrometry. The list of Schistosome sequences (from NCBI database) that matched the peptide masses on the bands is indicated with the corresponding ions scores. Ions score is 10 * Log ( ), where is the probability that the observed match is a random event. Individual ions scores >41 indicate identity or extensive homology ( ). The highlighted proteins are those previously recognized by pooled sera from adults.