Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan

Figure 1

Phylogenetic tree of COII sequences. Phylogenetic tree based on a fragment of 570 bp of COII gene of Leishmania parasite. Sequence dataset was analysed using MEGA 5, the neighbor-joining (NJ) method, and bootstrap analysis (1,000 replicates) based on the ClustalW algorithm. Significant bootstrapping values (>70%) are shown on the nodes. Only representative sequences were shown. Number indicates the sample number, HBM indicates human blood marrow origin sequence, HLN indicates lymph node origin sequence, and HB indicates human blood origin sequence used in this study. Blue square: sequence numbers 107 and 111 originated from skin culture amplificates and associated with CL patients; red circle: Leishmania GenBank reference sequence (L. donovani GenBank accession number AY660023, L. major GenBank accession number AF63316, and L. mexicana GenBank accession number HQ586845.1); green circle: our positive control L. infantum MHOM/TN/80/IPT1 used in this study.