Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan

Figure 4

Phylogenetic tree of ITS cloning sequences. Phylogenetic tree (unrouted) based on a fragment of 970 bp of ITS gene of Leishmania parasite. Sequence dataset was analyzed using MEGA 5, the neighbor-joining (NJ) method, and bootstrap analysis (1,000 replicates) based on the ClustalW algorithm. Significant bootstrapping values (>70%) are shown on the nodes. Blue square: colonies numbers 15 and 33 as representative sequences of 108 ITS cloned sample; pink triangle: colonies numbers 15, 18, 25, and 28 as representative sequences of 109 ITS cloned sample; purple lozenge: colonies numbers 2, 37, 39, and 40 as representative sequences of 110 ITS cloned sample; red circle: Leishmania GenBank reference sequence (L. donovani GenBank accession number AJ634357; L. major GenBank accession number FJ75339).