Molecular Characterization of Leptospira spp. in Environmental Samples from North-Eastern Malaysia Revealed a Pathogenic Strain, Leptospira alstonii

Table 1

List of primers used.

Primer	Targeted genes	Annealing temperature (°C)	Product size	Sequences	Sources
G1	secY		285 bp	5′-CTGAATCGCTGTATAAAAGT 3′	[11]
G2		55		5′-GGAAAACAAATGGTCGGAAG 3′
B64-I	flaB		563 bp	5′-CTGAATTCTCATCTCAACTC 3′
B64-II				5′-GCAAAATCAGATGGACG AT 3′
Pathogenic gene	LipL32	58	120 bp	5′-AGG TCT TTACAGAATTTCTTTCA 3′	This study
				5′-TTACTTAGTCGCGTCAGAA 3′
Sapro 1	rrs	55	240 bp	5′-AGAAATTTGTGCTAATACCGAATGT 3′	[12]
Sapro 2				5′-GGCGTCGCTGCTTCAGGCTTTCG 3′
Bak2-F	16S rRNA	52	796 bp	5′-AGTTTGATCMTGGCTCAG 3′	[13]
Bak2-R				5′-GGACTACHAGGGTATCTAAT 3′