A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae

Figure 1

Representative agarose gel electrophoresis of the multiplex PCR using reference strains of H. influenzae and K. pneumoniae. Lane M: 100 bp DNA ladder (Fermentas, USA); Lane 1: negative control (IC); Lane 2: PCR amplicon of K. pneumoniae; Lane 3: PCR amplicon of H. influenzae; Lane 4: PCR amplicons of multiplex PCR (K. pneumoniae + H. influenzae); Lane 5: PCR amplicons of multiplex PCR (IC + K. pneumoniae + H. influenzae).