A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae

Figure 2

Effect of different concentrations of trehalose on the performance of thermostabilized multiplex PCR. (a) 2% stabilizer. (b) 4% stabilizer. (c) 6% stabilizer. (d) 8% stabilizer. (e) 10% stabilizer. Lane M: 100 bp DNA ladder (Fermentas, USA); Lane 1: negative control before lyophilization (IC); Lane 2: positive control before lyophilization (IC + K. pneumoniae + H. influenzae); Lane 3: negative control after lyophilization (IC); Lane 4: positive control after lyophilization (IC + K. pneumoniae + H. influenzae).