A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae

Figure 3

Effect of different concentrations of Taq DNA polymerase on the performance of thermostabilized multiplex PCR. (a) 100% Taq DNA polymerase. (b) 150% Taq DNA polymerase. (c) 200% Taq DNA polymerase. (d) 250% Taq DNA polymerase. Lane M: 100 bp DNA ladder (Fermentas, USA); Lane 1: negative control before lyophilization (IC); Lane 2: positive control before lyophilization (IC + K. pneumoniae + H. influenzae); Lane 3: negative control after lyophilization (IC); Lane 4: positive control after lyophilization (IC + K. pneumoniae + H. influenzae).