A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae

Figure 6

Analytical sensitivity of thermostabilized multiplex PCR at bacterial level for H. influenzae and K. pneumoniae. Lane: M, 100 bp DNA ladder (Fermentas, USA); N, negative control (IC); 1, positive control (IC + K. pneumoniae + H. influenzae); 2, 10⁵ CFU/ml lysate of H. influenzae and K. pneumoniae; 3, 10⁴ CFU/ml lysate of H. influenzae and K. pneumoniae; 4, 10³ CFU/ml lysate of H. influenzae and K. pneumoniae; 5, 10² CFU/ml lysate of H. influenzae and K. pneumoniae; 6, 10¹ CFU/ml lysate of H. influenzae and K. pneumoniae.