A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae

Figure 7

(a) Accelerated stability evaluation at Day 0 storage of test at different temperatures. (A) 4°C. (B) 25°C. (C) 37°C. Lane M: 100 bp DNA ladder (Fermentas, USA); Lane 1: negative control (before thermostabilization); Lane 2: positive control (before thermostabilization); Lane 3: negative control (after thermostabilization); Lane 4: positive control (after thermostabilization). (b) Accelerated stability evaluation of test at different temperatures and storage periods. (A) Day 7. (B) Day 14. (C) Day 30. Lane M: 100 bp DNA ladder (Fermentas, USA); Lane 1: negative control at 4°C; Lane 2: positive control at 4°C; Lane 3: negative control at 25°C; Lane 4: positive control at 25°C; Lane 5: negative control at 37°C; Lane 6: positive control at 37°C.

(a)

(b)