Research Article
A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae
Table 1
Bacterial species and strains used in this study and results of the thermostabilized mPCR.
| | No | Reference strains | Php (202 bp) | P6 (582 bp) | Internal control (IC) |
| | (1) | Klebsiella pneumoniae | + | − | + | | (2) | Klebsiella pneumoniae | + | − | + | | (3) | Haemophilus influenzae | − | + | + | | (4) | Haemophilus influenzae | − | + | + | | Gram negative bacteria | | (5) | Pseudomonas aeruginosa | − | − | + | | (6) | Escherichia coli (EHEC) | − | − | + | | (7) | E. coli (ETEC) | − | − | + | | (8) | Shigella sonnei | − | − | + | | (9) | Shigella flexneri | − | − | + | | (10) | Shigella boydii | − | − | + | | (11) | Shigella dysenteriae | − | − | + | | (12) | Salmonella typhi | − | − | + | | (13) | Vibrio cholerae | − | − | + | | (14) | Citrobacter freundii | − | − | + | | (15) | Acinetobacter baumannii | − | − | + | | (16) | Acinetobacter spp. | − | − | + | | (17) | Klebsiella spp. | − | − | + | | (18) | Haemophilus parainfluenzae | − | − | + | | (19) | Proteus mirabilis | − | − | + | | (20) | Burkholderia pseudomallei | − | − | + | | (21) | Moraxella catarrhalis | − | − | + | | (22) | Citrobacter spp. | − | − | + | | (23) | Mycobacterium tuberculosis | − | − | + | | (24) | Mycobacterium bovis | − | − | + | | Gram positive bacteria | | (25) | Listeria monocytogenes | − | − | + | | (26) | Staphylococcus aureus | − | − | + | | (27) | Methicillin Resistant Staphylococcus aureus (MRSA) | − | − | + | | (28) | Streptococcus viridians | − | − | + | | (29) | Streptococcus pneumoniae | − | − | + | | (30) | Streptococcus pyogenes | − | − | + | | (31) | Streptococcus Group A | − | − | + | | (32) | Streptococcus Group B | − | − | + | | (33) | Streptococcus Group G | − | − | + | | (34) | Bacillus subtilis | − | − | + |
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strains from America Type Control Center (ATCC), USA.
of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia.
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