Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum

Figure 1

Analytical sensitivity of the conventional PCR and kDNA PCR-ELISA in L. infantum gDNA. (a) Analytical sensitivity of 6% polyacrylamide gel and (b) kDNA PCR-ELISA for the detection of PCR products from DNA extracted of L. infantum at concentrations of 1: 700 pg, 2: 70 pg, 3: 7 pg, 4: 700 fg, 5: 70 fg, 6: 7 fg, 7: 0.7 fg, 8: 0.07 fg, and 9: 0.007 fg/μL; NC: negative controls and M: 100 pb DNA Ladder (Promega).

(a)

(b)