Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum

Figure 3

Circulating L. infantum DNA levels measured using the kDNA PCR-ELISA in different groups. kDNA PCR-ELISA absorbance results for the detection of PCR products from DNA extracted from peripheral blood samples in different groups of patients with VL, VL/HIV, asymptomatic, and noninfected endemic controls. There was no statistically difference between Adult VL and VL/HIV Groups (). In contrast, the median (0,347) in the Asymptomatic Group was lower than the Children VL Group (). The dashed line represents the cut-off (0.096). Children VL Group: Median = 1.157, 25% Percentile = 0.886 and 75% Percentile = 1.345. Adult VL Group: Median = 1.057, 25% Percentile = 0.675 and 75% Percentile = 1.175. VL/HIV Group: Median = 1.099, 25% Percentile = 0.842 and 75% Percentile = 1.340. Asymptomatic Group: Median = 0.347, 25% Percentile = 0.255 and 75% Percentile = 0.531.