Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum

Table 1

Analytical specificity of the PCR detected in 6% polyacrylamide gels and the kDNA PCR-ELISA using DNA extracted from different Leishmania species.

DNA samples	Gel electrophoresis (kDNA)	kDNA ELISA
		Absorbance readings (450 nm)	Status
L. (L.) infantum	Positive	2,237	Positive
L. (L.) donovani	Positive	1,397	Positive
L. (V.) braziliensis	Positive	0,056	Negative
L. (V.) guyanensis	Positive	0,063	Negative
L. (L.) amazonensis	Positive	0,095	Negative