Research Article
Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida
Table 1
DENV NS1 detection in selected serum samples as determined by ELISA and in comparison to clinical molecular (qRT-PCR) and serological (anti-DENV IgM and IgG) results. The table below details the results of DENV NS1 detection by ELISA against qRT-PCR, IgM, and IgG DENV assays for a group of serum samples selected for inclusion and based on the following criteria: (1) denotes samples that were positive by qRT-PCR for DENV as determined by BOPHL-Tampa; (2) denotes samples that were positive for DENV by IgG detection only as determined by BOPHL-Tampa; (3) denotes samples that were DENV negative received by BOPHL-Tampa for all DENV-specific assays; (4) denotes samples that were collected from Martin County serosurvey and were found to be DENV negative by all DENV-specific assays. Index values represent the mean of duplicate values obtained when reading samples at 450 nm and taking calibrators into account. Negative samples had an index value > 0.9, those between 0.9 and 1.1 were equivocal, and those above 1.1 were positive for NS1 detection. Results are listed as either positive (+) or negative (neg) for each ELISA. Positive qRT-PCR results are reported either as neg or positive by listing serotype and value results. Samples that were qRT-PCR+ but NS1 neg are highlighted in bold font within the table. Note that no single assay here was capable of diagnosing DENV infection alone.
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