Research Article
Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida
Table 2
Breakdown of DENV serological diagnostic status (any combination of DENV NS1, anti-DENV IgM, and/or –IgG) versus detection of DENV RNA via qRT-PCR. The table below details first the comparison of DENV NS1 detection via ELISA compared to results obtained for the respective sample set via qRT-PCR (), set as a gold-standard. The table details a further breakdown of these results by including anti-DENV IgM and IgG status of the samples. Nine (9 out of 14) qRT-PCR+ samples were also DENV NS1+ (64.3%) and all 7 samples that were negative by qRT-PCR were also found to be negative for DENV NS1. Notably, all 5 DENV NS1− samples that were qRT-PCR+ were also anti-DENV IgM− and IgG+, while only 1 positive NS1 sample was found to have that same profile, indicating that nonprimary infections may affect the sensitivity of the DENV NS1 ELISA. note that 1 of the DENV qRT-PCR− samples was not assayed for DENV anti-IgG.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||