Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida

Table 3

Sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values for Panbio DENV NS1 ELISA when compared to detection of DENV RNA via qRT-PCR. Note that the “true negative” value of was left to stand in order to prevent skewing calculations in favor of qRT-PCR even though both assays failed to diagnose dengue correctly in 2 IgG+ samples.

	DENV qRT-PCR+ ()	DENV qRT-PCR− ()
DENV NS1 ELISA+	9	0	PPV = 100%
DENV NS1 ELISA−	5	7	NPV = 58.3%
	Sensitivity = 64.3%	Specificity = 100%