Loop-Mediated Isothermal Amplification Test for Trypanosoma gambiense Group 1 with Stem Primers: A Molecular Xenomonitoring Test for Sleeping Sickness

Table 1

The analysis of tsetse fly samples using T. brucei gambiense standard and stem LAMP test formats.

Source	Number of	Template type	LAMP tests
			Standard		Stem	value	Stem	value
Midgut	5	DNA	+	26	+	19	+	19
		Supernatant	+	23	+	15	+	15
	10	DNA	−	—	+	21	+	21
		Supernatant	+	25	+	18	+	18
	15	DNA	−	—	−	—	−	—
		Supernatant	−	—	+	24	+	24
	20	DNA	−	—	−	—	−	—
		Supernatant	−	—	−	—	−	—
Whole fly	1	DNA	+	28	+	20	+	20
		Supernatant	+	26	+	19	+	20
	5	DNA	−	—	−	—	−	—
		Supernatant	−	—	+	—	+	23
	10	DNA	−	—	−	—	−	—
		Supernatant	−	—	−	—	+	28^†

G. pallidipes (midguts or whole fly).
TgsGP LAMP test [24].
threshold (minutes).
out of 6 replicates showed the predicted product.