Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea

Table 1

Nucleotide sequences for Cryptosporidium hominis and parvum for stem SAM-1 LAMP test. Degenerate primers have been underlined and made bold.

Target	Primer name	Sequence (5′-3′)	Bases	Final amplicon size
SAM-1	F3	GAGGATGGGGTGCTCATGG	19	220 bp
	B3	CCTTATTAACTATCTCCAGYAG	22
	FIP	GACTTTGCAACAAGYCTTGCCAGCATTTAGCGGGAAAGATG	41
	BIP	ATTGGAATAGCAARGCCTTTATCGTCATTATACCCATCTTTCGC	44
	LF	CRCCTGAYCTATCTACTTTAG	21
	LB	CTRTATATTAATACATTTGGCAC	23
	SF	TACACAAKCCAGAAAAGACG	20
	SB	TGTTTGGTRCAGGTTTCATATG	20
	Probe	CTTGTGTAGCAGATGTTTGGTACAGG	26