Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea

Table 2

The analytical sensitivity of stem LFD LAMP test formats, SAM-1 LAMP, and PCR tests using a 10-fold serial dilution of C. hominis DNA.

Test	Primer combination	Probe	10-fold serial dilution						Result (Min)	Remarks
					10^−5	10^−6	10^−7	10^−8
Stem LFD LAMP	F3/B3, FIP/BIP, LF/LB, SF/SB	FITC	+	+	+	+	±	−	30	This study
Stem LFD LAMP	FIP/BIP, LF/LB, SF/SB	FITC	+	±	−	−	−	−	40	This study
LAMP	F3/B3, LF/LB, FIP/BIP	N/A	+	±	−	−	−	−	40	This study
SAM-1 LAMP	F3/B3, LF/LB, FIP/BIP	N/A	+	+	+	−	−	−	35	[15]
Nested PCR test	F1/R1; F2/R2	N/A	+	+	+	−	−	−	N/A	[31]

LAMP test with outer F3/B3 primers; LAMP test without outer F3/B3 primers. Primers designed in this study. The reaction done using 10⁻⁴ (1000 oocysts/ml). Time to recording a positive reaction. Approximately 30% of the replicates were consistently positive with preheated template and sequencing; N/A: not applicable.