Table 2: The analytical sensitivity of stem LFD LAMP test formats, SAM-1 LAMP, and PCR tests using a 10-fold serial dilution of C. hominis DNA.

TestPrimer combinationProbe10-fold serial dilutionResult (Min)Remarks
10−510−610−710−8

Stem LFD LAMPF3/B3, FIP/BIP, LF/LB, SF/SBFITC++++±30This study
Stem LFD LAMPFIP/BIP, LF/LB, SF/SBFITC+±40This study
LAMPF3/B3, LF/LB, FIP/BIPN/A+±40This study
SAM-1 LAMPF3/B3, LF/LB, FIP/BIPN/A+++35[15]
Nested PCR testF1/R1; F2/R2N/A+++N/A[31]

LAMP test with outer F3/B3 primers; LAMP test without outer F3/B3 primers. Primers designed in this study. The reaction done using 10−4 (1000 oocysts/ml). Time to recording a positive reaction. Approximately 30% of the replicates were consistently positive with preheated template and sequencing; N/A: not applicable.