Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea

Table 3

The comparative analysis of stem LFD LAMP, SAM-1 LAMP, and nested PCR test in the detection of previously confirmed and archived C. hominis and C. parvum DNA samples and clinical samples.

	Indices	Types of test
		Stem LFD-LAMP	SAM-1 LAMP	Nested PCR
Cryptosporidium spp. DNA ( = 39)	Number of positive samples	29 (74.4%)	27 (69.2%)	25 (64.1%)
Clinical samples ( = 67)	Number of positive samples	16 (23.9%); 19 (28.4%)	14 (20.8%)	11 (16.4%)
	Time to results (Min)	80	120	320
	Accelerating primers	Loop and stem	Loop	nd

SAM-1 LAMP test [15]; SSU rRNA nested PCR [31]. Template was preheated for 5 minutes. Detection using gel electrophoresis. From master mix preparation to visual result readout. The samples were also positive using stem LFD LAMP test; Nd: not done.