Table 4: Practice of malaria detection.

ParametersMedical technologist
Freq.%
Laboratory
scientist
Freq.%
Others
Freq.%
Total
Freq.%

Does thick blood smear with Giemsa require fixing technique with absolute methanol?75 (78.1)11 (91.7)8 (80)94 (79.7)
Does thick blood film with a lesser amount of blood lead to misinterpreting results?80 (83.3)11 (91.7)8 (80)99 (83.9)
Should blood smear be air-dried before fixing?92 (95.8)11 (91.7)9 (90)112 (94.9)
Should the nucleus of white blood cells be dark blue in case of good quality of Giemsa staining?71 (74)8 (66.7)8 (80)87 (73.7)
Do pure Giemsa require stirring before filtering and filtering before using every time?86 (89.6)9 (75)10 (100)105 (89)
Does destaining require slowly rinsing the glass slide with tap water to completely destain?85 (88.5)11 (91.7)9 (90)105 (89)
Should the Giemsa diluent be neutral at pH 7.2?91 (94.8)11 (91.7)10 (100)112 (94.9)
Should pure Giemsa be stored in a brown bottle and tightly screwed closed to protect against evaporation and oxidation reaction?84 (87.5)10 (83.3)10 (100)105 (89)
Should detection of malaria by blood film technique be done via objective lens?86 (89.6)10 (83.3)10 (100)106 (89.8)
Does thin blood smear require well margins of spreader and suitable drop of blood?91 (94.8)9 (75)9 (90)109 (92.4)
Mean score (X±SD)8.35±1.068.17±1.598.5±1.358.34±1.14