LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae)

Table 2

Number of revertant colonies of Salmonella typhimurium TA98 and TA100 tester strains induced by ethanolic extracts of D. multiflora and P. pinnata.

Plant	Concentration (mg/mL)	His + revertants plate
		TA98	TA100
D. multiflora	5	23.00 ± 0.57 (1.131)	135.00 ± 1.00 (1.298)
	0.5	21.66 ± 2.66 (1.065)	120.66 ± 2.33 (1.160)
	0.005	14.33 ± 0.33 (0.704)	133.00 ± 2.00 (1.278)
P. pinnata	5	19.00 ± 0.00 (0.934)	142.33 ± 2.33 (1.368)
	0.5	14.33 ± 0.33 (0.704)	139.33 ± 2.33 (1.339)
	0.005	7.33 ± 0.33 (0.360)	116.00 ± 0.00 (1.115)
4 NQO		128.00 ± 0.66 (2 µg/mL)	389.33 ± 1.33 (1 µg/mL)
10% DMSO		22.00 ± 0.57 (1.082)	98.12 ± 0.33 (0.919)
Water		20.33 ± 0.88 (1)	101.00 ± 3.00 (1)

The positive control used in this study was 4-nitroquinoline 1-oxide (4-NQO) (Sigma) at concentrations of 2 and 1 μg/mL for S. typhimurium TA98 and TA100, respectively. His: histidine; DMSO: dimethylsuphoxide (negative control); H₂O: water (negative control). All cultures were made in triplicate (except the solvent control where five replicates were made). The results are expressed as a mean number of revertants ± standard deviation and mutagenic index values (in parentheses).