New Genetic Variants of Leptospira spp Characterized by MLST from Peruvian Isolates

Table 1

List of MLST loci, primers, and amplification conditions used for the typing of pathogenic Leptospira spp, based on the method described by Boonsilp et al., 2013 with modifications.

Loci	Primers	Nucleotide sequence (5´ a 3´)	MgCl2 (mM)	Primers concentration (μM)	PCR size (pb)	loci size (pb)	Localization in chromosome I
pntA	pntA-FM	TAG GAA ARA TGA AAC CRG GAA C	3.5	0.2	621	525	56347–56871
	pntA-RM	AAG AAG CAA GAT CCA CAA YTA C
caiB	caiB-F	CAACTTGCGGAYATAGGAGGAG	3.5	0.2	650	402	1562845–1563246
	caiB-R	ATTATGTTCCCCGTGAYTCG
glmU	glmU-FM	AGG ATA AGG TCG CTG TGG TA	3.5	0.2	650	444	3784955–3784512
	glmU-RM	AGT TTT TTT CCG GAG TTT CT
tpiA	pntA-FM	TTG CAG GAA ACT GGA AAA TGA AT	3.5	0.2	639	426	1694673–1694248
	pntA-RM	GTTTTACRGAACCHCCGTAGAGAAT
pfkB	pfk B-FM	CGGAGAGTTTTATAARAAGGACAT	1.5	0.2	588	432	1386553–1386984
	pfk B-RM	AGAACACCCGCCGCAAAACAAT
sucA	sucA-FM	TCA TTC CAC TTY TAG ATA CGA T	3.5	0.6	640	447	1227474–1227920
	sucA-RM	TCTTTTTTGAATTTTTGACG
mreA	mreA-FM	GGC TCG CTC TYG ACG GAA A	3.5	0.6	719	435	2734550–2734116
	mreA-RM	TCCRTAACTCATAAAMGACAAAGG