Development of a Competitive Enzyme-Linked Immunosorbent Assay Based on Purified Recombinant Viral Protein 7 for Serological Diagnosis of Epizootic Haemorrhagic Disease in Camels

Figure 2

(a) Coomassie staining of SDS-PAGE of recVP7 purified. Lane 1, uninfected Sf9 used as negative control; lane 2, recVP7 purified from the pellet; lane 3, rec VP7 purified from the supernatant. Molecular weight marker, fragment sizes are measured in kDa (M). (b) Western blotting analysis of recVP7 purified, using anti-VP7 mAb clone 4G11 HRP-conjugated. lane 1, uninfected Sf9 used as negative control; lane 2, rec VP7 purified from pellet; lane 3, recVP7 purified from the supernatant. Molecular weight marker, fragment sizes are measured in kDa (M). (c) Western blotting analysis of recVP7 purified using anti-V5 mAb HRP-conjugated. Lane 1, recVP7 purified from the pellet; lane 2, recVP7 purified from the supernatant; lane 3, uninfected Sf9 used as negative control. Molecular weight marker, fragment sizes are measured in kDa (M). All the samples were fractioned by the SDS-PAGE under denaturing conditions.