A Novel Multiplex LAMP Assay for the Rapid and Accurate Diagnosis of Visceral Leishmaniasis Caused by Leishmania infantum from Iran

Table 6

Results of testing molecular tools on the clinical samples.

Samples	Real-time PCR	Nested PCR	LAMP1	LAMP2	Multiplex LAMP
50 negatives	—	—	—	1 positive	2 positives
55 positives	48 positives, 7 negatives	45 positives, 10 negatives	35 positives, 20 negatives	50 positives, 5 negatives	55 positives, 0 negative
	(Sensitivity 87% and specificity 100%)	(Sensitivity 81% and specificity 100%)	(Sensitivity 63% and specificity 100%)	(Sensitivity 90% and specificity 98%)	(Sensitivity 100% and specificity 96%)