Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis
Figure 7
Demonstration of CD1d dependence of iNKT cells. Proliferation of line 1F1.1 in response to thyroglobulin was assessed after blocking CD1d. Different concentrations of mouse CD1d mAb were used starting from 0.0–3.0 μg/well. Concentration 0 represents no blocking. Proliferation in response to thyroglobulin was abrogated after CD1d blocking showing that iNKT cells recognized thyroglobulin in a CD1d-dependent manner. [3H] thymidine incorporation was measured as an indicator of proliferative response. Data represents mean CPM of triplicate wells from each concentration.
