Thyroid Hormone and Cardiac Disease: From Basic Concepts to Clinical Application
Figure 4
Structural and phenotypic effects of T3 supplementation. After 10 days of culture, T3-supplemented myocardial fragments presented better preserved cardiomyocytes both at histological (a) and immunohistochemical examination (c). Weaker staining and progressive atrophy was observed in the corresponding untreated samples ((b) and (d), resp.). Myocardial sections were stained hematoxylin eosin (a), (b) for total protein content evaluation or immunostained for α-sarcomeric actinin (c), (d) for sarcomeric specif protein content. Images were acquired with a CCD camera and optical density was measured (scale bar = 50 μm). (e) Quantification of the histological results obtained from hematoxylin-eosin and α-sarcomeric-actinin staining of 10-day-old human myocardium in culture. P was determined using the two-tailed unpaired Student’s t-test; data are expressed as mean ± SEM (experimental data derived from [28]).
