FRTL-5 Rat Thyroid Cells Release Thyroglobulin Sequestered in Exosomes: A Possible Novel Mechanism for Thyroglobulin Processing in the Thyroid

Table 1

Uncalibrated optical density (OD) measurements of the Tg bands from Figure 3 corresponding to exosome pellets are presented here. The “sample applied” refers to the total volume applied to the gel and “sample amount” refers to the volume of the original dissolved exosome pellet present within that applied sample. Optical density measurements are normalized to the sample volumes and the ratio of Tg optical density to total protein concentration of the exosome samples is calculated and represented here multiplied by a factor of 1000.

Treatment	OD	Sample applied (μL)	Sample amount (μL)	OD/sample	Protein (μg/mL)	Ratio
5H culture 1	0.184	15	7.5	0.368	50.22	7.328
6H culture 1	0.261	15	7.5	0.522	36.69	14.227
5H culture 2	0.085	15	3.5	0.364	48.50	7.505
6H culture 2	0.134	15	3.5	0.574	36.82	15.589