Modulating Thyroid Hormone Levels in Adult Mice: Impact on Behavior and Compensatory Brain Changes

Figure 6

Gene expression following TH pertubation. RNA was extracted from brain and the effect of treatment on gene expression of a select set of genes was tested by qPCR. Several genes related to TH activation/inactivation, transport, and use were significantly affected by thyroxine treatment. (a) Dio2 trended toward a decrease with thyroxine treatment, whereas the inactivating Dio3 was robustly expressed (b). Expression of the two brain-specific TH transporters, Slc16a2 (c) and Slc01c1 (d) were not significantly changed by either treatment. Expression of both TH receptors (Thra (e) and Thrb (f)) was reduced compared with control-treated mice. (g) Transthyretin (Ttr) expression was also reduced in thyroxine-treated mice. Although there were not significant changes in expression of these genes with PTU/Met treatment, Dio2 expression tends to increase compared with control. compared with control. The dashed line represents the control group, all standardized to 1. Control = 7 M/6 F, thyroxine = 7 M/6 F, and PTU/Met = 5 M/8 F.

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