Modulating Thyroid Hormone Levels in Adult Mice: Impact on Behavior and Compensatory Brain Changes

Table 1

Sex-specific changes due to treatment.

ASSAY/TEST	CONTROL		THYROXINE		PTU/MET
	MALES	FEMALES	MALES	FEMALES	MALES	FEMALES
SERUM T4 (µg/dL)	8.881 ± 1.144	8.79 ± 1.69	98.04 ± 12.59^A	89.63 ± 6.62^A	1.33 ± 0.5	1.41 ± 0.38
SERUM T3 (µg/mL)	2.26 ± 0.47	3.05 ± 0.34	3.88 ± 0.85	3.29 ± 1.66	1.25 ± 0.18	4.55 ± 1.17
BRAIN T4 (fg/mg tissue)	768.3 ± 20.38	648.9 ± 25.8	885.1 ± 39	790.5 ± 62.9^A	782.8 ± 33.1	676.8 ± 12.6
BRAIN T3 (fg/mg tissue)	18.51 ± 3.41	23.82 ± 4.03	33.45 ± 3.95	22.17 ± 5.06	16.3 ± 6.26	42.84 ± 12.41
HEART (mg)	216.4 ± 16	162 ± 6.4	320.8 ± 17.4^A	285.4 ± 18.5^A	165.8 ± 21.2	133.3 ± 9.8
FOOD (g)	3.1 ± 0.1	3.1 ± 0.06	7.2 ± 1.2	5.5 ± 0.2^A	3.4 ± 1	2.3 ± 0.1
WATER (mL)	6.4 ± 1.3	6.3 ± 0.6	15.6 ± 4.4	11.9 ± 0.2^A	6.9 ± 3.2	4.2 ± 0.7
OPEN FIELD
Distance (cm)	3943 ± 270	4194 ± 279	3748 ± 268	4771 ± 165	1780 ± 312^A	2729 ± 243^A
Time in center (s)	35.1 ± 8.1	44.5 ± 16.6	70.5 ± 15.5	63 ± 17.4	37 ± 13.6^A	36.8 ± 8.1
EPM
Immobility (s)	159.8 ± 9.8	147 ± 11.9	158.1 ± 7.1	133.5 ± 5.2	227.8 ± 14.2^A	179 ± 15.3
Open arms (s)	29.3 ± 5.8	42.9 ± 11.4	95.5 ± 18.2^A	95.1 ± 6.9^A	6.6 ± 2.3	20.2 ± 3.5
qPCR (fold change)
Dio2	0.95 ± 0.13	1.06 ± 0.1	0.67 ± 0.2	0.55 ± 0.09^A	1.56 ± 0.49	1.17 ± 0.12
Dio3	1.1 ± 0.06	0.89 ± 0.06	10.1 ± 1.92^A	13.9 ± 3.63^A	1.03 ± 0.05	1.11 ± 0.14
Slc16a2	1.08 ± 0.09	0.9 ± 0.16	0.74 ± 0.06^A	0.92 ± 0.1	1.32 ± 0.1	0.89 ± 0.12
Slc01c1	0.88 ± 0.07	1.14 ± 0.1	0.95 ± 0.09	0.99 ± 0.05	1.06 ± 0.07	1.05 ± 0.09
Thra	1.06 ± 0.04	0.93 ± 0.07	0.78 ± 0.05^A	0.72 ± 0.05^A	1.23 ± 0.08	0.95 ± 0.03
Thrb	0.94 ± 0.07	1.08 ± 0.05	0.82 ± 0.05	0.92 ± 0.06	1.02 ± 0.09	0.96 ± 0.04
Ttr	1.02 ± 0.07	0.98 ± 0.05	0.81 ± 0.06	0.89 ± 0.06	1.18 ± 0.06	0.92 ± 0.06

^acompared with control.