Xenotransplantation of Human Neural Progenitor Cells to the Subretinal Space of Nonimmunosuppressed Pigs

Figure 1

Identification of donor cells, graft placement, and survival at 10 days. Donor cells were identified via anti-human nuclear immunolabeling and an FITC-conjugated secondary antibody. Abundant human nuclear profiles (green) extend in a line horizontally across the image, the location of which is the subretinal space, corresponding precisely to the initial site of deposition. In this particular view, there is no evidence of migration or integration of donor cells into host tissues. The retina, seen here as the faintly striated, unlabelled tissue directly beneath the grafted cells, appears detached from the overlying structures; however, this was not seen in vivo and is an artifact of the method of histological processing employed.