Xenotransplantation of Human Neural Progenitor Cells to the Subretinal Space of Nonimmunosuppressed Pigs
Figure 5
Evaluation of host tissue and cellular response in the area of laser burn. A subset of sections from eyes with xenografts were stained with H&E (a) to evaluate the host tissue and cellular responses, while adjacent sections were immunolabeled to identify human donor cells (b). (a) The area of laser injury can be identified by the focal disturbance of the overlying pigmented layers, as well as localized disruption of the underlying retina. An abnormal adhesion between the retina and overlying structures is present in this location that bridges the subretinal space. A dense mononuclear infiltrate is present in the choroid (top), consistent with the development of a strong immune response. (b) Immunoimaging reveals the presence of many donor cells, which have preferentially congregated in the area of laser injury and appear to contribute to the subretinal adhesion. A few donor profiles are present in the neural retina in the immediate vicinity of the laser injury. Survival = 10 days.
