Figure 1: Overexpression of miR-21 regulates endogenous Pdcd4 and Pclo mRNAs. (a) MIN6 cells were treated 24 hours with cytokine cocktail IL-1β (50 U/mL), TNF-α (2000 U/mL), and IFNγ (100 U/mL). The expression of miR-21 was assessed by qRT-PCR. (b) Overexpression of miR-21 mimic (300 nM) for 48 hs inhibits the expression of endogenous Pdcd4 and Pclo mRNA. Experiments shown in (a) and (b) are expressed as mean ± SD ( 𝑛 = 5 ), * 𝑃 < 0 . 0 5 ( 𝑡 -test, 2 tails). (c) miR-21 recognition site in the 3′UTR of Pclo splicing version 2. MiR-21 “seed” is shown in red font. (d) Semiquantitative RT-PCR shows expression of both Pclo versions (V1, V2) in MIN6 insulinoma cells.